Hjem Publikasjoner Molecular scaffolds underpinning macrogli[...]

Molecular scaffolds underpinning macroglial polarization: An analysis of retinal Muller cells and brain astrocytes in mouse

Vit. artikkel

Publisert

  • 2012

Key roles of macroglia are inextricably coupled to specialized membrane domains. The perivascular endfoot membrane has drawn particular attention, as this domain contains a unique complement of aquaporin‐4 (AQP4) and other channel proteins that distinguishes it from perisynaptic membranes. Recent studies indicate that the polarization of macroglia is lost in a number of diseases, including temporal lobe epilepsy and Alzheimer's disease. A better understanding is required of the molecular underpinning of astroglial polarization, particularly when it comes to the significance of the dystrophin associated protein complex (DAPC). Here, we employ immunofluorescence and immunogold cytochemistry to analyze the molecular scaffolding in perivascular endfeet in macroglia of retina and three regions of brain (cortex, dentate gyrus, and cerebellum), using AQP4 as a marker. Compared with brain astrocytes, Müller cells (a class of retinal macroglia) exhibit lower densities of the scaffold proteins dystrophin and α‐syntrophin (a DAPC protein), but higher levels of AQP4. In agreement, depletion of dystrophin or α‐syntrophin—while causing a dramatic loss of AQP4 from endfoot membranes of brain astrocytes—had only modest or insignificant effect, respectively, on the AQP4 pool in endfoot membranes of Müller cells. In addition, while polarization of brain macroglia was less affected by dystrophin depletion than by...

Enger, Rune; Gundersen, Georg Andreas; Haj-Yasein, Nadia Nabil; Eilert-Olsen, Martine; Thoren, Anna; Vindedal, Gry Fluge; Petersen, Petur Henry; Skare, Øivind; Nedergaard, Maiken; Ottersen, Ole Petter; Nagelhus, Erlend Arnulf
Glia 60(12): 2018–2026
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