Chemistry, biomarkers, and particle characterisation

  • Inductively coupled plasma optical emission spectrometer (ICP-OES)
  • Inductively coupled plasma mass spectrometer (ICP-QQQ MS)
  • Liquid chromatography tandem mass spectrometers (LC-MS/MS)
  • Liquid chromatography with UV-Vis/fluorescence detection (LC-UV/FL)
  • Field emission scanning electron microscope (FE-SEM)
  • Scanning Mobility Particle Sizer (SMPS)
  • Aerodynamic Particle Sizer (APS)
  • Aerosol monitors
  • Gass chromatography Flame Ionisation (GC-FID)
  • Proton-transfer-reaction mass spectrometer (PTR-MS)
  • Thermal Optical Aerosol Analyzer (OC-EC Aerosol Analyzer)
  • Ion Chromatography with conductivity detection (IC)
  • Fourier transform infrared spectrometer (FT-IR)
  • X-ray Diffraction Spectrometer (XRD)
  • Field Flow Fractionation (FFF)
  • Spectrophotometer
  • Limulus amoebocyte lysate (LAL) – Endotoxin determination
  • NOx – Analyzer
  • Ozone – Analyzer
  • Real-Time Fiber Monitor
  • Direct reading gas monitors
  • Aersol samplers:
    • MOUDI impactor
    • Inhalable aerosol samplers (GSP, IOM, Respicon, CIS, PAS6)
    • Thoracic aerosol sampler (BGI GK22.69 cyclones, Respicon)
    • Respirable aerosol samplers (JS Holdings cyclones, Respicon)
    • Diesel Particulate Matter cyclones (GK SCC0.695)
    • Coriolis µ – Microbial air sampler

Molecular biology

  • Western Blot – Protein Analysis and Detection
  • Nanodrop (ND) – DNA/RNA/Protein quantification and purity
  • BioAnalyzer: DNA/RNA/Protein quality
  • Qubit DNA/RNA/Protein- DNA/RNA/Protein quantification (higher specificity and sensitivity)
    QiaCube – automated RNA/DNA isolation
  • nCounter Sprint (NanoString) – gene expression determination
  • Digital Droplet Polymerase Cain Reaction (ddPCR) – direct quantification and clonally amplify nucleic acids (DNA, cDNA or RNA)
  • Air-Liquid Interface laboratory (ALI-lab), including Cloud system 6, Cloud system 12 and AES system (Automated Exposure System)
  • Incucyte Live Cell Analysis system – capture cellular changes where they happen – in the incubator
  • Automatic cell counter – Used for cell count and viability when seeding cells for experiments
    Quantification PCR (qPCR)
  • StepOnePlus™ Real-Time PCR System – used for quantitative Real-Time PCR results for a variety of genomic research applications
  • QuantStudio 5 – Real time PCR system
  • Peltier Thermal cycler – run PCR
  • Eppendorf Mastercycler x50s – run PCR
  • Qiagen Pyromark Q48 – Pyrosequencing
  • Dynamic Light Scattering (DLS): measures the hydrodynamic diameter of nanoparticles in solution and provides information on the aggregation state of nanoparticles in solution
  • Nanoparticle Tracking Analysis (Nanosight): instruments that visualize, characterize and measure small particles in suspension
  • Phase contrast microscope – Used for cell culture maintenance
  • Fluorescence microscope – For cells, microbes and tissue labeled with fluorescent antibodies
    Light microscope – for tissue morphology and differential cell count
  • Confocal microscope – For cells and tissue labelled with fluorescent antibodies, can stack images to form 3 dimensional images (X, Y and Z direction)
  • Calcium microscope
  • Covaris M220 sonicator
  • Rotary microtome
  • Cryomicrotome
  • Tissue processor and paraffin embedding station
  • Flow Cytometry (CytoFlex) – analyse single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution
  • MagPix Bio-Plex – analysis of biomarkers in biological samples
  • xMAP intelliflex – analysis of biomarkers in biological samples
  • Ultra freezer (-80°C)
  • Cryofreezer
  • Synergy neo2 – Spectrophotometer for plates

Physical exposures

  • Sound Meter
  • VR-goggles
  • Climate chamber – Enables a temperature-controlled (down to -20°C) testing environment
  • Medoc instrumentation with CPM (conditioned pain modulation) protocol and CHEPs (Contact Heat Evoked Potentials)
  • Scantainers – Temperature-controlled housing environments for rodents

Health outcomes

  • Spirometry
  • Vibrametry
  • Gas exchange measurement (DLCO)
  • Measurement equipment for quantification of tremor and hand steadiness
    METAMAX 3B – for portable metabolic testing
  • FLIR – Thermography camera
  • IButtons – Temperature loggers
  • Cognitive testing (50 tablets): Incorporating PVT (Psychomotor vigilance task) and CANTAB (Cambridge Neuropsychological Testing Automated Battery)
  • 32 or 64 channel EEG (Electroencephalography): Utilized to study brain activity and neurological responses
  • Equivital measurements: Covering ECG, heart rate, skin temperature, telemetry, breathing rate, activity, and body position
  • Oura rings (60 rings) and Axivity sensors: Recording movement, skin temperature, heart rate variability, blood oxygen rate for determination of e.g. sleep length and quality
  • GE Logiq S7 expert – Utrasound Machine
  • TANITA MC-780MA – body composition scale
  • Dinamap V100 – Vital Signs Monitor